Bifunctional P-450/nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 TYR-166   ARG-167  16.7 16.1 8.6 -16.3 36.1 35.7 11.6
 ARG-167   ASP-168  14.5 13.8 7.5 -0.4 36.0 29.1 -47.8
 ASP-168   GLN-169  15.0 13.0 -41.4 26.5 114.7 90.1 -38.2
 GLN-169   PRO-170  11.2 9.9 -35.4 -0.1 39.2 18.4 142.3
 PRO-170   HIS-171  8.3 7.8 4.3 15.0 129.3 122.7 -0.2
 HIS-171   PRO-172  7.4 7.4 -6.8 9.0 113.4 110.6 -20.7
 PRO-172   PHE-173  5.9 6.3 -9.1 -0.2 70.1 70.5 24.1
 PHE-173   ILE-174  2.9 3.3 -1.8 1.4 127.5 124.5 -9.2

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 LYS-224   ALA-225  5.4 5.2 -2.8 3.6 116.0 122.2 -19.6
 ALA-225   SER-226  8.5 8.3 -30.0 13.4 112.8 119.8 -55.3
 GLN-229   SER-230  6.8 5.2 0.1 -0.2 138.1 129.0 -44.0
 SER-230   ASP-231  6.8 7.2 20.3 49.8 79.2 98.6 -80.4
 ASP-231   ASP-232  8.0 9.7 -89.1 56.3 27.9 40.4 151.4
 ASP-232   LEU-233  8.2 7.6 -16.2 -12.6 72.7 100.2 9.3
 LEU-233   LEU-234  7.8 7.4 17.0 -1.3 76.8 70.8 26.6
 LEU-234   THR-235  4.1 3.8 -4.5 -5.9 163.0 177.1 -36.8
 THR-235   HIS-236  4.3 4.0 -4.1 12.9 101.7 112.5 13.8
 HIS-236   MET-237  7.7 7.4 -6.2 -10.1 63.2 80.9 3.7
 HIS-236   MET-237  7.7 7.4 -6.2 -10.1 63.2 80.9 3.7

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 HIS-236   MET-237  7.7 7.4 -6.2 -10.1 63.2 80.9 3.7
 HIS-236   MET-237  7.7 7.4 -6.2 -10.1 63.2 80.9 3.7
 MET-237   LEU-238  7.3 7.2 4.3 16.9 54.6 53.0 19.9
 LEU-238   ASN-239  5.6 5.0 -4.2 34.0 161.3 159.6 122.4

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees