Beta-Galactosidase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASP-746   PHE-747  11.1 11.2 0.3 3.9 28.6 26.3 84.6  PHE-747   CYS-748  8.2 8.4 4.0 0.6 111.3 111.5 -62.0  CYS-748   ILE-749  7.1 7.2 2.3 -0.7 45.0 45.7 48.7  ILE-749   GLU-750  5.0 5.0 -3.8 4.0 72.1 72.6 1.0  GLU-750   LEU-751  7.6 7.5 4.3 -1.5 55.9 55.3 6.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  TRP-756   GLN-757  3.9 3.8 -2.9 3.9 84.9 82.8 22.9  GLN-757   PHE-758  5.7 5.6 1.6 -4.6 74.8 77.2 -2.5  PHE-758   ASN-759  6.4 6.6 4.6 -3.1 84.8 85.6 56.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-763   PHE-764  11.1 11.3 -0.2 5.8 67.6 68.4 -50.0  PHE-764   LEU-765  7.9 8.1 -5.0 7.0 99.6 101.4 -35.4  LEU-765   SER-766  4.2 4.4 -4.8 -1.9 30.6 30.6 114.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLN-775   LEU-776  6.1 6.1 4.1 4.6 56.6 59.2 95.2  LEU-776   LEU-777  3.2 3.1 -6.6 5.9 129.4 130.7 -6.4  LEU-777   THR-778  4.0 4.0 -0.3 -0.8 84.6 85.6 -101.2  THR-778   PRO-779  4.0 4.1 -3.8 2.6 27.9 27.6 117.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees