Citrate-Sodium Symporter

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-136   LEU-137  15.2 17.3 -26.1 -11.8 46.9 52.8 70.1  LEU-137   LEU-138  16.4 17.0 0.7 3.2 99.8 64.1 -20.1  LEU-138   LYS-139  13.9 15.2 21.1 -3.4 115.0 85.6 -23.8  LYS-139   SER-140  10.5 12.8 -12.2 -4.8 25.0 29.4 30.3  SER-140   LEU-141  12.0 12.2 34.0 -11.2 130.6 107.7 -43.1  LEU-141   LEU-142  12.5 10.8 19.9 -12.4 91.8 77.1 7.0  LEU-142   GLY-143  9.5 7.5 -17.1 -29.1 59.4 52.5 69.6  GLY-143   TYR-144  7.1 6.5 -37.0 13.3 17.4 24.7 70.1  TYR-144   ILE-145  9.4 9.1 -3.3 -11.0 52.8 47.2 21.1 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-246   VAL-247  15.0 14.8 17.2 11.5 141.5 150.5 -63.1  VAL-247   ARG-248  13.8 14.1 -28.8 -13.5 68.2 68.3 50.8  ARG-248   LYS-249  14.4 13.8 -46.5 -46.1 22.1 6.6 266.5  ASP-257   GLU-258  25.3 24.1 -149.0 31.7 107.2 110.2 -116.4  GLU-258   LYS-259  28.2 27.6 12.5 -15.2 42.7 19.3 1.8  LYS-259   ALA-260  30.9 30.0 -38.2 11.6 97.2 77.4 2.3  ALA-260   GLY-261  33.8 33.3 26.2 -42.3 102.2 51.4 -27.3  GLY-261   GLN-262  37.4 36.6 116.6 0.7 28.7 65.9 227.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PHE-360   ALA-361  7.4 8.7 43.3 -23.4 44.3 62.2 49.8  ALA-361   ASN-362  4.6 5.2 0.8 3.4 38.1 27.4 12.3  ASN-362   VAL-363  7.5 6.9 6.4 9.3 53.0 54.6 36.0  VAL-363   VAL-364  9.0 9.1 1.5 3.0 53.8 55.9 23.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees