Pyruvate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LYS-85   GLY-86  4.5 4.9 0.8 -15.5 120.5 122.7 35.1  GLY-86   PRO-87  1.6 2.0 12.1 -10.7 91.4 104.3 9.0  PRO-87   GLU-88  4.7 5.0 -65.8 28.1 40.7 40.3 116.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASP-185   LEU-186  11.9 12.1 -13.2 -17.6 25.3 33.4 112.3  LEU-186   PRO-187  9.7 9.5 -16.6 -4.4 57.3 74.4 29.9  PRO-187   ALA-188  8.6 8.0 -8.3 -1.4 102.1 100.7 -12.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees