Bifunctional P-450/nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 PHE-165   TYR-166  16.3 16.1 3.4 -5.0 73.4 73.4 -10.9
 TYR-166   ARG-167  16.7 16.5 2.8 -10.8 35.0 34.8 23.4
 ARG-167   ASP-168  14.2 13.9 10.8 7.4 42.3 40.8 -67.6
 ASP-168   GLN-169  14.6 12.8 -45.2 25.8 109.4 86.9 -22.0
 GLN-169   PRO-170  10.7 9.4 -30.7 -10.9 32.4 11.8 172.8
 PRO-170   HIS-171  8.2 7.8 14.6 7.7 123.7 119.1 -52.4
 HIS-171   PRO-172  7.2 7.3 -3.7 12.6 111.9 106.9 15.2
 PRO-172   PHE-173  5.0 5.7 -12.0 0.4 63.2 65.8 8.0

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LYS-224   ALA-225  6.9 6.3 -2.5 9.1 108.1 114.5 -20.4
 ALA-225   SER-226  10.4 9.9 -30.5 15.9 112.3 116.9 -56.9
 GLN-229   SER-230  8.7 6.6 -2.2 -10.2 48.2 56.3 41.3
 SER-230   ASP-231  7.9 7.4 10.6 28.5 71.2 77.4 -7.6
 ASP-231   ASP-232  7.9 8.3 -79.7 73.1 35.2 22.4 43.3
 ASP-232   LEU-233  8.0 7.7 -6.7 -10.7 77.0 83.5 -13.4

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees