Reverse Transcriptase/ribonuclease H

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-214   THR-215  19.3 17.5 -11.4 -21.7 112.6 122.5 -20.1  TYR-232   GLU-233  6.9 1.7 -14.4 22.8 147.9 44.7 2.1  GLU-233   LEU-234  4.8 2.2 16.2 5.9 92.7 150.2 -22.3  LEU-214   THR-215  33.3 29.7 -11.4 -21.7 68.2 37.5 30.3  TYR-232   GLU-233  8.8 25.2 -14.4 22.8 90.4 83.4 -11.6  GLU-233   LEU-234  9.9 23.3 16.2 5.9 29.5 118.6 -7.3  LEU-234   HIS-235  10.3 19.5 39.9 -26.0 85.3 121.8 -8.2  LEU-214   THR-215  19.3 17.5 -11.4 -21.7 112.6 122.5 -20.1  TYR-232   GLU-233  6.9 1.7 -14.4 22.8 147.9 44.7 2.1  GLU-233   LEU-234  4.8 2.2 16.2 5.9 92.7 150.2 -22.3  LEU-214   THR-215  33.3 29.7 -11.4 -21.7 68.2 37.5 30.3  TYR-232   GLU-233  8.8 25.2 -14.4 22.8 90.4 83.4 -11.6  GLU-233   LEU-234  9.9 23.3 16.2 5.9 29.5 118.6 -7.3  LEU-234   HIS-235  10.3 19.5 39.9 -26.0 85.3 121.8 -8.2  HIS-235   PRO-236  11.8 18.3 -81.2 -14.4 162.5 83.1 -36.6  PRO-236   ASP-237  13.2 14.8 13.9 59.3 65.9 137.5 -22.6  ASP-237   LYS-238  11.2 15.1 -44.3 105.2 68.1 41.8 172.7  LYS-238   TRP-239  8.1 15.0 -165.2 29.2 56.3 97.2 -211.6  TRP-239   THR-240  6.5 11.7 -36.0 -6.6 32.3 65.5 22.5  THR-240   VAL-241  5.4 8.6 -12.8 44.8 63.0 81.5 -12.2  VAL-241   GLN-242  4.7 5.8 13.1 -35.5 140.9 137.4 23.2  GLN-242   PRO-243  7.5 7.4 -59.7 -9.4 33.9 68.5 29.1  PRO-243   ILE-244  6.3 4.5 11.1 49.7 115.0 118.0 -29.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PRO-313   VAL-314  6.0 3.0 50.3 42.1 37.0 87.9 58.8  VAL-314   HIS-315  3.6 4.3 -0.5 64.9 132.9 118.1 33.9  HIS-315   GLY-316  4.0 7.8 -36.0 -137.2 149.9 134.9 -124.0  GLY-316   VAL-317  2.8 9.0 174.1 17.8 47.7 48.1 178.9  VAL-317   TYR-318  4.5 5.8 -47.6 -38.3 129.5 122.2 -42.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees