Bifunctional P-450/nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PRO-172   PHE-173  4.3 3.3 -27.7 7.1 67.6 81.4 31.1  PHE-173   ILE-174  1.1 0.5 -7.3 12.4 135.7 146.8 17.7  ILE-174   THR-175  1.4 2.4 -6.0 -2.2 129.4 126.1 -24.2  THR-175   SER-176  1.7 1.2 -3.0 -5.4 66.8 67.1 5.9  SER-176   MET-177  4.1 3.9 14.0 -16.8 93.6 100.6 24.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASN-213   ASP-214  5.0 5.1 26.9 -16.2 62.2 68.3 33.9  ASP-214   LEU-215  4.6 4.5 5.8 -33.3 88.3 79.6 -35.4  LEU-215   VAL-216  1.6 1.5 33.4 -4.7 44.5 54.3 74.1  VAL-216   ASP-217  1.6 1.7 14.1 -16.7 25.4 29.6 -2.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees