M-Calpain

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-220   VAL-221  4.6 5.2 -10.6 -43.4 103.4 79.4 -15.1  VAL-221   THR-222  2.7 4.3 6.0 29.4 101.2 60.6 7.1  THR-222   GLU-223  2.2 3.0 -3.0 68.2 127.8 132.7 85.5  GLU-223   TRP-224  5.2 5.2 -5.9 9.1 70.7 71.0 -2.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees