Pyruvate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ARG-90   THR-91  4.9 4.1 -12.1 7.0 79.2 75.3 38.3  THR-91   GLY-92  7.0 6.8 -9.1 3.3 45.0 45.2 7.7  GLY-92   GLN-93  9.7 9.5 1.2 -16.3 63.0 67.2 -19.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  TYR-125   ILE-126  9.3 9.1 21.9 -21.5 119.9 113.2 3.6  ILE-126   ASP-127  5.6 5.4 7.1 25.0 36.1 37.2 104.2  ASP-127   TYR-128  3.2 2.4 -57.0 35.6 97.1 109.4 -34.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-135   ARG-136  11.2 11.6 -0.2 -23.2 89.1 89.1 -5.8  ARG-136   PRO-137  14.6 14.9 3.8 2.8 52.0 34.2 9.8  PRO-137   GLY-138  16.1 16.2 13.2 -7.3 26.7 33.2 24.2 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ARG-174   ARG-175  10.1 9.8 15.3 -52.9 149.4 142.6 114.4  ARG-175   GLY-176  8.4 7.9 20.0 1.0 76.1 81.3 -11.3  GLY-176   VAL-177  5.6 4.6 -0.1 3.8 71.5 74.8 -14.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees