Dna-Directed RNA Polymerase II 140Kd Polypeptide

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLU-1149   ARG-1150  3.4 4.2 -17.5 9.9 31.4 37.1 73.5  ARG-1150   LEU-1151  1.6 2.4 29.3 -28.7 86.3 82.9 -18.7  LEU-1151   MET-1152  2.6 1.7 6.4 2.3 66.0 63.4 50.3  MET-1152   GLU-1153  2.8 2.4 -31.9 31.0 47.8 50.1 41.0  GLU-1153   ALA-1154  6.0 5.9 64.3 -8.3 64.7 78.1 133.5  ALA-1154   SER-1155  7.8 9.0 -137.6 100.9 105.2 152.0 -175.0  SER-1155   ASP-1156  10.4 10.8 105.3 -109.7 38.9 49.9 70.8  ASP-1156   ALA-1157  11.3 12.0 29.3 -20.8 23.9 31.2 56.4  ALA-1157   PHE-1158  9.7 10.1 -38.7 18.0 127.3 137.5 -126.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees