Pyruvate Kinase Isozymes M1/m2
(All numbering and residues are taken from first PDB file)
Bending Residue Dihedral Analysis
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
THR-113
LYS-114
5.8
5.7
4.3
-10.6
136.7
138.3
19.8
LYS-114
GLY-115
6.2
6.1
5.1
-16.1
112.3
113.7
14.6
GLY-115
PRO-116
3.1
3.1
19.2
-3.8
94.1
107.7
-21.7
PRO-116
GLU-117
5.3
5.3
-87.6
62.5
21.2
32.6
90.4
GLU-117
ILE-118
7.6
6.8
4.7
-14.9
95.9
90.5
4.0
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
ASP-216
LEU-217
11.9
11.9
-12.5
-1.2
44.6
45.6
55.6
LEU-217
PRO-218
9.4
9.1
-8.3
-15.7
51.3
70.3
53.0
PRO-218
ALA-219
7.8
7.4
-2.4
4.8
107.4
100.9
20.0
ALA-219
VAL-220
5.4
4.5
-26.3
24.8
74.2
50.6
-20.3
VAL-220
SER-221
2.8
2.8
-15.9
-22.6
75.2
60.2
79.1
SER-221
GLU-222
5.1
4.9
13.7
-6.2
159.9
151.7
-46.7
GLU-222
LYS-223
6.4
5.7
-6.4
8.3
86.7
96.9
11.9
LYS-223
ASP-224
6.8
6.7
-7.5
-4.7
91.6
100.0
-1.6
ASP-224
ILE-225
3.5
3.5
8.5
-12.0
152.7
145.7
13.2
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees