Dna-Directed RNA Polymerase II 140Kd Polypeptide

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ILE-1103   HIS-1104  25.8 25.6 136.5 -131.9 76.1 73.7 -3.9  HIS-1104   ALA-1105  25.1 24.1 6.0 -3.9 96.7 91.3 -28.5  ALA-1105   ARG-1106  21.9 21.4 49.1 -57.7 79.8 77.0 -63.3  ARG-1106   ALA-1107  21.6 21.0 38.7 -13.5 98.8 88.8 35.0  ALA-1107   ARG-1108  19.6 19.1 -5.8 163.5 83.3 103.1 137.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees