Twitching Motility Protein Pilt

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  SER-111   LEU-112  3.6 4.3 14.3 -29.5 45.3 52.6 -39.6  LEU-112   PRO-113  4.4 4.9 -22.8 36.9 138.1 149.4 40.9  PRO-113   ALA-114  6.3 6.8 -4.2 0.7 144.9 140.8 -27.9  ALA-114   GLU-115  4.7 5.0 -36.7 54.5 150.2 149.5 96.4  GLU-115   ILE-116  7.5 7.9 4.1 18.9 86.5 104.3 -67.9  ILE-116   PRO-117  10.1 10.2 10.5 -21.1 76.1 76.7 -36.7  PRO-117   GLU-118  13.5 13.6 -12.5 5.9 152.1 145.0 -16.2 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees