Inducible Nitric Oxide Synthase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ILE-427   MET-428  4.6 4.3 15.8 -66.5 80.0 71.7 -10.7  MET-428   ASP-429  0.8 1.2 49.4 22.5 22.0 56.6 99.2  ASP-429   HIS-430  0.7 3.0 -26.7 -16.5 87.7 68.2 6.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-458   VAL-459  21.9 13.9 -169.6 33.7 120.9 4.2 -192.5  VAL-459   PRO-460  19.0 14.9 -26.7 21.3 122.8 75.5 -21.6  PRO-460   PRO-461  19.0 12.9 23.8 -17.8 123.4 112.0 -16.4  PRO-461   VAL-462  19.8 15.2 -148.4 -92.2 107.6 3.7 262.9  VAL-462   SER-463  17.9 14.1 173.8 135.0 52.4 101.0 -11.5  SER-463   GLY-464  21.2 13.4 47.1 41.8 12.8 61.4 -391.8  GLY-464   SER-465  20.0 16.1 -80.3 48.4 113.7 71.8 520.1  SER-465   ILE-466  17.4 17.5 -17.6 12.1 97.0 70.0 3.2  ILE-466   THR-467  20.2 14.3 -129.0 7.0 154.1 132.6 -154.3  THR-467   PRO-468  21.2 13.4 21.1 11.8 91.7 67.5 19.6  PRO-468   VAL-469  24.5 15.6 -151.7 60.3 135.4 144.2 -112.9  VAL-469   PHE-470  24.0 15.9 -163.6 47.7 91.5 78.8 9.2  PHE-470   HIS-471  27.1 18.2 -136.4 1.3 109.5 87.9 167.2  HIS-471   GLN-472  26.8 20.8 -158.5 -69.0 93.0 142.5 -241.3  GLN-472   GLU-473  30.0 20.8 13.1 -148.6 135.2 44.7 68.8  GLU-473   MET-474  32.9 23.3 149.2 -2.6 141.4 30.1 66.6  MET-474   LEU-475  31.2 21.9 29.5 -37.2 83.7 41.5 -13.6  LEU-475   ASN-476  29.5 22.9 29.6 3.4 145.3 59.6 -1.0  ASN-476   TYR-477  26.5 21.1 -57.6 -20.3 95.1 165.5 -113.9  TYR-477   VAL-478  23.7 20.9 27.7 22.2 100.0 58.7 5.4  VAL-478   LEU-479  20.1 18.7 -40.5 -3.0 85.3 134.5 -28.9  LEU-479   SER-480  16.9 17.4 58.8 8.9 42.7 48.1 78.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees