Periplasmic Substrate Binding Protein

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 GLN-62   SER-63  5.6 5.6 -6.3 13.3 18.4 17.2 -65.3
 SER-63   PRO-64  6.1 5.8 -15.4 -2.5 51.8 54.6 34.9
 GLN-62   SER-63  13.5 13.2 -6.3 13.3 61.1 56.2 -18.2
 SER-63   PRO-64  10.2 9.8 -15.4 -2.5 8.3 10.0 52.3
 PRO-64   GLY-65  9.5 9.6 -1.1 -9.6 72.2 61.7 31.2
 GLN-62   SER-63  17.4 17.2 -6.3 13.3 93.5 90.5 6.9
 SER-63   PRO-64  13.7 13.5 -15.4 -2.5 28.9 24.7 79.8
 PRO-64   GLY-65  11.1 11.6 -1.1 -9.6 83.4 70.8 24.0
 GLN-62   SER-63  5.6 5.6 -6.3 13.3 18.4 17.2 -65.3
 SER-63   PRO-64  6.1 5.8 -15.4 -2.5 51.8 54.6 34.9
 GLN-62   SER-63  13.5 13.2 -6.3 13.3 61.1 56.2 -18.2
 SER-63   PRO-64  10.2 9.8 -15.4 -2.5 8.3 10.0 52.3
 PRO-64   GLY-65  9.5 9.6 -1.1 -9.6 72.2 61.7 31.2
 GLY-65   PHE-66  10.1 8.6 1.4 -3.3 78.7 87.0 -16.5
 GLN-62   SER-63  17.4 17.2 -6.3 13.3 93.5 90.5 6.9
 SER-63   PRO-64  13.7 13.5 -15.4 -2.5 28.9 24.7 79.8
 PRO-64   GLY-65  11.1 11.6 -1.1 -9.6 83.4 70.8 24.0
 GLY-65   PHE-66  10.8 9.5 1.4 -3.3 52.0 59.5 -16.3
 GLY-65   PHE-66  10.1 8.6 1.4 -3.3 78.7 87.0 -16.5
 GLY-65   PHE-66  10.8 9.5 1.4 -3.3 52.0 59.5 -16.3
 GLN-62   SER-63  5.6 5.6 -6.3 13.3 18.4 17.2 -65.3
 SER-63   PRO-64  6.1 5.8 -15.4 -2.5 51.8 54.6 34.9
 GLN-62   SER-63  13.5 13.2 -6.3 13.3 61.1 56.2 -18.2
 SER-63   PRO-64  10.2 9.8 -15.4 -2.5 8.3 10.0 52.3
 PRO-64   GLY-65  9.5 9.6 -1.1 -9.6 72.2 61.7 31.2
 GLY-65   PHE-66  10.1 8.6 1.4 -3.3 78.7 87.0 -16.5
 GLN-62   SER-63  17.4 17.2 -6.3 13.3 93.5 90.5 6.9
 SER-63   PRO-64  13.7 13.5 -15.4 -2.5 28.9 24.7 79.8
 PRO-64   GLY-65  11.1 11.6 -1.1 -9.6 83.4 70.8 24.0
 GLY-65   PHE-66  10.8 9.5 1.4 -3.3 52.0 59.5 -16.3
 GLY-65   PHE-66  10.1 8.6 1.4 -3.3 78.7 87.0 -16.5
 GLY-65   PHE-66  10.8 9.5 1.4 -3.3 52.0 59.5 -16.3

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 PHE-66   THR-67  10.9 10.5 66.2 -51.5 86.0 112.4 -1.8
 THR-67   GLY-68  9.2 9.8 43.9 -1.7 30.4 45.2 257.0
 GLY-68   SER-69  10.0 11.9 -13.0 4.8 93.5 74.3 -74.5
 PHE-66   THR-67  13.6 12.3 66.2 -51.5 54.8 71.7 52.6
 THR-67   GLY-68  13.8 13.7 43.9 -1.7 14.4 2.2 185.9
 GLY-68   SER-69  11.9 13.2 -13.0 4.8 130.6 118.4 -47.0
 PHE-66   THR-67  14.0 12.9 66.2 -51.5 35.2 46.1 103.8
 THR-67   GLY-68  13.9 13.5 43.9 -1.7 48.3 33.6 197.7
 GLY-65   PHE-66  10.1 8.6 1.4 -3.3 78.7 87.0 -16.5
 GLY-65   PHE-66  10.8 9.5 1.4 -3.3 52.0 59.5 -16.3
 PHE-66   THR-67  10.9 10.5 66.2 -51.5 86.0 112.4 -1.8
 THR-67   GLY-68  9.2 9.8 43.9 -1.7 30.4 45.2 257.0
 GLY-68   SER-69  10.0 11.9 -13.0 4.8 93.5 74.3 -74.5
 GLY-65   PHE-66  10.1 8.6 1.4 -3.3 78.7 87.0 -16.5
 PHE-66   THR-67  13.6 12.3 66.2 -51.5 54.8 71.7 52.6
 THR-67   GLY-68  13.8 13.7 43.9 -1.7 14.4 2.2 185.9
 GLY-68   SER-69  11.9 13.2 -13.0 4.8 130.6 118.4 -47.0
 GLY-65   PHE-66  10.8 9.5 1.4 -3.3 52.0 59.5 -16.3
 PHE-66   THR-67  14.0 12.9 66.2 -51.5 35.2 46.1 103.8
 THR-67   GLY-68  13.9 13.5 43.9 -1.7 48.3 33.6 197.7
 GLY-65   PHE-66  10.1 8.6 1.4 -3.3 78.7 87.0 -16.5
 GLY-65   PHE-66  10.8 9.5 1.4 -3.3 52.0 59.5 -16.3
 PHE-66   THR-67  10.9 10.5 66.2 -51.5 86.0 112.4 -1.8
 THR-67   GLY-68  9.2 9.8 43.9 -1.7 30.4 45.2 257.0
 GLY-68   SER-69  10.0 11.9 -13.0 4.8 93.5 74.3 -74.5
 GLY-65   PHE-66  10.1 8.6 1.4 -3.3 78.7 87.0 -16.5
 PHE-66   THR-67  13.6 12.3 66.2 -51.5 54.8 71.7 52.6
 THR-67   GLY-68  13.8 13.7 43.9 -1.7 14.4 2.2 185.9
 GLY-68   SER-69  11.9 13.2 -13.0 4.8 130.6 118.4 -47.0
 GLY-65   PHE-66  10.8 9.5 1.4 -3.3 52.0 59.5 -16.3
 PHE-66   THR-67  14.0 12.9 66.2 -51.5 35.2 46.1 103.8
 THR-67   GLY-68  13.9 13.5 43.9 -1.7 48.3 33.6 197.7

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 PHE-78   ILE-79  2.8 3.1 -4.4 -7.0 72.8 81.0 -3.9
 ILE-79   PRO-80  1.0 0.7 10.6 9.3 21.2 24.0 112.2
 PRO-80   TYR-81  3.0 3.0 -11.9 -6.2 101.3 98.2 -46.2
 TYR-81   LEU-82  4.8 4.7 10.7 18.5 113.0 123.4 -74.7

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 TRP-208   PHE-209  9.4 9.5 -29.7 15.4 46.1 46.9 -5.7
 PHE-209   THR-210  9.2 9.2 -17.5 30.3 65.3 57.0 -70.6
 TRP-208   PHE-209  4.3 4.0 -29.7 15.4 119.1 124.3 -97.7
 TRP-208   PHE-209  9.4 9.5 -29.7 15.4 46.1 46.9 -5.7
 PHE-209   THR-210  9.2 9.2 -17.5 30.3 65.3 57.0 -70.6
 SER-205   HIS-206  3.4 3.2 -12.4 5.1 169.2 165.8 -27.4
 HIS-206   GLY-207  3.1 3.1 -15.2 46.9 111.1 110.9 37.9
 GLY-207   TRP-208  0.8 1.1 -32.6 -6.1 49.6 47.9 136.2
 TRP-208   PHE-209  4.3 4.0 -29.7 15.4 119.1 124.3 -97.7

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 ARG-280   ALA-281  10.7 11.2 -7.2 13.5 112.3 114.4 14.8
 ALA-281   PRO-282  7.5 7.7 7.3 -0.5 22.3 13.8 59.3
 PRO-282   GLN-283  8.5 8.6 1.3 5.6 40.8 52.6 11.2

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees