Alcohol Dehydrogenase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-175   PHE-176  4.2 4.1 7.3 -10.1 83.4 89.8 -44.9  PHE-176   SER-177  2.9 2.8 17.6 -24.3 152.9 150.4 -1.8  SER-177   THR-178  2.0 2.1 4.4 1.4 135.6 139.3 15.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ALA-317   ILE-318  4.7 4.8 -7.4 1.2 40.4 45.6 79.6  ILE-318   PHE-319  3.3 3.8 8.6 -2.1 125.1 128.1 -32.3  PHE-319   GLY-320  1.2 1.7 -3.7 2.5 91.9 94.1 48.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLU-357   GLY-358  18.7 19.0 -9.3 4.5 68.8 65.2 21.3  GLY-358   PHE-359  15.5 15.7 -15.7 10.0 34.5 31.9 -0.1  PHE-359   ASP-360  17.6 17.5 16.7 -22.6 72.9 77.0 -65.0  ASP-360   LEU-361  19.8 19.9 8.3 5.9 83.4 72.5 125.9  LEU-361   LEU-362  17.0 17.3 -0.4 6.0 32.3 35.2 -55.7  LEU-362   ARG-363  15.9 16.2 -13.9 14.8 64.6 66.3 14.8  ARG-363   SER-364  19.5 19.8 7.9 6.5 65.8 58.7 130.6  SER-364   GLY-365  20.7 21.2 -14.9 27.6 93.3 88.2 14.0  GLY-365   GLU-366  17.6 18.8 18.1 -0.7 42.5 30.7 201.3  GLU-366   SER-367  16.8 17.2 -113.2 68.5 110.5 88.1 -165.0  SER-367   ILE-368  13.3 13.5 41.6 -28.2 144.2 141.0 -178.0  ILE-368   ARG-369  10.8 11.1 -30.4 34.0 71.1 79.9 -63.5  ARG-369   THR-370  8.4 8.4 1.2 2.9 41.3 31.1 28.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees