2-Isopropylmalate Synthase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ALA-424   VAL-425  16.3 16.9 -7.6 -4.9 102.8 105.0 -6.5  VAL-425   ILE-426  13.1 13.8 5.9 8.5 14.4 19.0 11.8  LYS-434   GLY-435  9.0 9.3 -29.8 56.1 107.4 125.4 17.5  GLY-435   GLY-436  9.2 11.1 133.9 -19.1 134.7 109.2 173.5  GLY-436   VAL-437  10.4 10.9 -98.5 -8.4 86.9 83.1 45.9  VAL-437   ALA-438  14.2 14.6 4.4 -9.4 134.7 132.3 2.6  ALA-438   TYR-439  15.0 15.0 7.7 -4.7 80.2 74.6 -4.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees