Pyruvate Kinase, M2 Isozyme

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LYS-114   GLY-115  8.0 7.9 -9.6 -11.0 69.4 68.8 50.1
 GLY-115   PRO-116  5.9 6.2 22.6 -8.1 109.8 123.0 -25.5
 PRO-116   GLU-117  8.0 8.4 -82.2 81.0 13.9 29.2 -6.7
 GLU-117   ILE-118  9.7 9.3 -18.5 -1.8 75.6 70.3 98.3

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LEU-217   PRO-218  12.1 12.6 -1.9 -9.6 35.7 47.8 22.5
 PRO-218   ALA-219  10.9 11.1 -5.7 -6.4 98.4 97.6 27.4
 ALA-219   VAL-220  7.4 7.6 -1.2 2.2 82.8 50.5 7.2
 VAL-220   SER-221  6.7 7.2 -48.4 9.7 91.2 75.7 25.0
 SER-221   GLU-222  9.6 10.3 -1.8 8.8 25.3 26.2 -9.3
 GLU-222   LYS-223  11.6 12.0 -8.5 7.6 84.5 92.0 -4.7

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees