Fusion Protein Beta-2 Adrenergic Receptor/lysozyme

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  SER-204   ILE-205  1.5 1.7 2.6 7.1 22.9 17.1 38.1  ILE-205   VAL-206  3.9 3.4 6.4 1.0 67.0 66.7 11.3  VAL-206   SER-207  6.6 6.2 -4.4 18.3 97.4 97.8 13.9  SER-207   PHE-208  6.3 6.2 -33.0 17.2 114.9 128.6 -40.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLU-268   HIS-269  18.5 17.0 5.9 -10.4 58.8 24.0 -27.6  HIS-269   LYS-270  16.5 18.8 -24.1 10.0 144.0 135.2 -57.1  LYS-270   ALA-271  19.3 20.9 14.0 -20.9 38.6 70.7 7.5  ALA-271   LEU-272  18.8 18.0 6.9 -12.0 66.1 60.7 -12.4  LEU-272   LYS-273  15.0 15.5 14.4 3.4 55.2 29.9 67.9  LYS-273   THR-274  15.9 18.2 3.5 1.9 25.7 47.9 -6.3  THR-274   LEU-275  18.1 17.3 -11.5 6.0 129.5 117.1 -11.0  LEU-275   GLY-276  15.4 13.5 13.3 -7.8 73.8 50.8 31.0  GLY-276   ILE-277  12.9 14.8 -8.3 6.3 141.3 148.4 1.0  ILE-277   ILE-278  15.4 16.9 -13.4 7.7 148.7 118.9 -34.3  ILE-278   MET-279  15.0 14.3 -22.6 5.6 111.2 121.2 -48.0  MET-279   GLY-280  11.2 11.1 5.2 2.9 71.6 45.7 13.9  GLY-280   THR-281  12.0 13.7 -32.6 20.3 152.5 154.0 -40.4  THR-281   PHE-282  14.3 15.4 18.7 -11.2 40.4 57.0 18.5  PHE-282   THR-283  11.7 12.0 -17.4 4.3 101.2 120.0 -20.8  THR-283   LEU-284  9.3 10.5 3.1 17.4 59.3 46.6 61.5  LEU-284   CYS-285  12.3 13.9 -10.0 -3.3 156.4 151.3 -56.3  CYS-285   TRP-286  12.9 13.3 -1.5 -10.3 129.4 120.4 -35.6  TRP-286   LEU-287  9.3 9.5 3.9 -4.9 89.6 87.0 -6.5  LEU-287   PRO-288  7.9 8.3 -1.8 14.2 131.7 143.2 44.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees