RNA Polymerase II Mediator Complex Subunit 20

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LYS-3   SER-4  8.0 7.6 159.6 -11.5 22.3 25.2 1616.9  SER-4   ALA-5  6.1 5.8 -5.2 10.1 92.1 85.2 40.6  ALA-5   VAL-6  5.4 5.6 -7.8 0.2 167.9 165.0 -102.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-9   GLU-10  12.5 12.6 -4.1 15.4 43.6 54.5 -73.1  GLU-10   ARG-11  14.0 14.2 -17.6 2.1 54.1 40.5 119.0  ARG-11   ALA-12  17.2 17.1 19.1 -14.2 115.8 116.1 47.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  TRP-35   SER-36  7.4 7.3 -0.6 6.6 103.5 111.9 -44.1  SER-36   ILE-37  4.1 4.4 -7.6 -12.3 40.6 41.3 159.8  ILE-37   ASP-38  3.3 3.2 -8.1 12.7 108.9 107.4 23.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-124   ASP-125  1.2 1.1 -8.4 11.9 61.6 64.0 -39.2  ASP-125   ALA-126  1.7 1.9 3.4 -12.6 96.9 94.4 -42.6  ALA-126   GLY-127  5.4 5.5 9.3 -3.0 36.8 43.1 92.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ILE-153   GLU-154  7.8 7.8 -6.0 11.6 41.1 45.2 -69.2  GLU-154   LEU-155  6.2 6.0 -0.8 -0.4 63.4 61.6 12.4  LEU-155   GLN-156  8.6 8.4 -23.4 18.3 50.4 52.7 60.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASP-158   GLU-159  14.6 13.7 10.3 43.0 9.4 28.9 622.8  GLU-159   ALA-160  14.3 14.8 -28.3 -2.8 103.1 119.9 -166.9  ALA-160   GLY-161  11.1 12.1 18.4 -5.3 59.8 62.5 25.5  GLY-161   GLU-162  13.6 14.7 148.1 136.8 123.1 118.5 470.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LYS-183   THR-184  12.3 12.6 1.1 -13.2 104.9 102.8 82.7  THR-184   SER-185  9.9 10.1 -2.4 -1.3 53.5 44.3 -1.3  SER-185   SER-186  6.7 7.0 14.3 -14.0 109.9 104.7 -13.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  TYR-204   VAL-205  3.5 3.9 0.7 -3.4 88.9 86.4 23.2  VAL-205   ARG-206  7.0 7.3 0.6 3.8 64.5 64.2 37.1  ARG-206   ALA-207  7.8 8.1 5.3 -15.2 123.0 126.0 15.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees