Pyruvate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ARG-90   THR-91  4.4 3.5 -13.2 7.3 77.1 74.4 23.9  THR-91   GLY-92  6.5 6.4 -12.2 2.3 41.2 43.0 25.6  GLY-92   GLN-93  9.2 8.9 3.7 -19.6 66.0 69.4 -33.1 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  TYR-125   ILE-126  8.8 8.8 17.7 -11.7 124.1 114.5 -2.4  ILE-126   ASP-127  5.3 5.1 13.6 25.2 41.1 38.5 128.4  ASP-127   TYR-128  2.5 2.0 -55.5 29.6 94.0 107.2 -34.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-135   ARG-136  11.7 12.0 3.0 -32.3 92.1 90.2 3.8  ARG-136   PRO-137  15.1 15.2 3.5 -0.4 53.5 35.9 0.2  PRO-137   GLY-138  16.2 16.4 8.8 -3.8 23.2 32.1 26.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASP-173   ARG-174  12.9 12.2 1.0 -11.6 99.2 85.2 13.2  ARG-174   ARG-175  9.5 9.3 18.9 -51.3 154.3 143.8 98.9  ARG-175   GLY-176  8.0 7.5 27.1 -4.1 80.6 83.6 -5.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees