Macromolecule-Binding Periplasmic Protein

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PRO-131   ASP-132  7.5 7.2 -0.5 -9.1 66.1 65.1 26.3  ASP-132   GLY-133  6.7 6.5 -31.6 48.9 72.1 64.0 -14.9  GLY-133   VAL-134  4.3 3.5 30.3 -2.5 38.1 52.3 70.3  VAL-134   VAL-135  4.3 3.5 -15.3 -21.7 86.9 107.1 4.4  VAL-135   ALA-136  4.4 3.4 31.0 -11.1 88.2 91.5 6.0  ALA-136   ARG-137  4.2 4.2 4.9 -2.8 116.7 114.9 2.1 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASP-307   SER-308  7.2 6.9 12.1 -6.0 62.4 64.9 9.8  SER-308   ARG-309  4.1 3.5 -1.2 1.9 85.3 93.9 -5.3  ARG-309   GLN-310  0.3 0.4 -17.8 -5.9 36.6 36.1 62.1  GLN-310   LYS-311  2.0 2.4 -0.1 14.3 109.4 102.6 18.8  LYS-311   VAL-312  5.5 5.6 -13.4 -11.5 78.5 85.5 21.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ARG-397   GLN-398  3.5 3.4 0.4 2.7 155.0 155.7 -16.0  GLN-398   TRP-399  5.0 5.1 -2.6 -1.2 77.5 77.9 6.1  TRP-399   THR-400  4.9 5.3 -1.7 10.7 108.3 113.4 9.1  THR-400   THR-401  2.4 2.9 -12.3 -6.3 35.8 35.1 50.9  THR-401   PRO-402  3.4 4.2 11.6 -7.2 84.2 85.4 8.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees