T7 RNA Polymerase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ALA-225   THR-226  6.1 11.0 21.6 -16.6 133.4 113.9 -9.4  THR-226   ARG-227  9.0 14.4 14.0 27.2 127.2 123.8 -20.4  ARG-227   ALA-228  9.0 13.9 131.4 12.1 116.9 150.5 176.5  ALA-228   GLY-229  8.3 11.1 -128.4 -110.6 117.8 48.5 -42.1  GLY-229   ALA-230  6.0 13.6 -116.6 7.0 145.4 58.9 -45.4  ALA-230   LEU-231  7.0 16.2 -14.3 16.9 110.8 50.8 -4.2  LEU-231   ALA-232  5.7 14.1 -53.1 -44.1 67.0 26.9 76.1  ALA-232   GLY-241  9.0 12.5 72.5 -21.6 66.3 128.0 -9.9  GLY-241   ILE-242  11.2 15.9 -14.4 -6.6 127.2 66.8 -9.3  ILE-242   SER-243  8.9 17.1 -67.7 -22.5 60.2 60.2 69.9  SER-243   PRO-244  10.0 14.1 -104.0 -16.4 125.1 28.4 -161.2  PRO-244   MET-245  11.0 14.5 0.7 -42.3 68.1 130.7 -8.6  MET-245   PHE-246  14.0 12.3 110.9 72.4 126.9 146.2 118.2  PHE-246   GLN-247  16.1 15.1 18.4 -3.2 158.8 155.3 -14.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees