Importin Subunit Alpha-3

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLN-241   GLU-242  11.1 11.1 -7.3 2.5 19.4 15.4 20.4  GLU-242   ILE-243  10.1 10.7 9.3 -13.4 89.0 98.3 -11.8  ILE-243   LEU-244  7.0 7.4 1.3 6.0 73.9 75.6 46.4  LEU-244   PRO-245  5.6 5.6 -4.9 2.1 46.1 50.1 -25.2  PRO-245   ALA-246  6.2 5.8 -10.3 5.0 118.2 117.3 -24.9  PRO-245   ALA-246  6.2 5.8 -10.3 5.0 118.2 117.3 -24.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PRO-245   ALA-246  6.2 5.8 -10.3 5.0 118.2 117.3 -24.9  PRO-245   ALA-246  6.2 5.8 -10.3 5.0 118.2 117.3 -24.9  ALA-246   LEU-247  3.0 2.8 1.3 1.0 127.0 134.8 -42.1  LEU-247   CYS-248  1.0 1.4 -7.5 -6.8 88.3 74.8 21.6  CYS-248   VAL-249  4.3 3.0 0.8 9.8 41.7 46.3 52.4  VAL-249   LEU-250  3.9 3.6 3.3 8.3 103.2 96.9 14.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-260   ASP-261  9.4 10.0 -3.7 -5.3 90.7 90.2 -10.6  ASP-261   THR-262  8.2 8.8 15.2 -13.9 86.8 91.8 -10.8  THR-262   VAL-263  4.5 5.1 3.0 8.9 18.9 20.0 63.2 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees