Serine/threonine-Protein Kinase Pak 4

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-362   LEU-363  9.7 9.0 -12.3 -2.4 66.6 67.8 8.9  LEU-363   PHE-364  6.4 5.6 -5.3 2.9 92.1 110.8 7.9  PHE-364   ASN-365  5.0 4.4 2.7 -3.0 138.9 120.0 10.1  ASN-365   GLU-366  7.7 7.4 -5.5 -6.7 25.9 24.7 35.0  GLU-366   VAL-367  5.9 5.9 17.6 -4.2 99.9 88.3 -21.1  VAL-367   VAL-368  2.8 3.4 2.8 -7.2 94.7 75.9 -8.9  VAL-368   ILE-369  6.2 6.6 -12.1 -1.7 33.3 45.1 42.7  ILE-369   MET-370  7.8 8.9 0.4 -20.2 143.0 149.4 38.5  MET-370   ARG-371  5.3 7.2 17.5 5.5 82.7 96.1 40.9  ARG-371   ASP-372  6.5 6.7 9.5 -14.1 103.9 115.3 -3.7  ASP-372   TYR-373  9.9 10.4 24.5 10.5 150.1 143.0 -88.0  TYR-373   GLN-374  10.1 10.5 47.5 -42.6 142.2 138.1 -10.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-378   VAL-379  6.7 6.9 -5.2 -5.1 86.3 82.6 25.7  VAL-379   GLU-380  3.2 3.3 -11.8 3.5 51.9 37.8 48.8  GLU-380   MET-381  2.7 3.3 5.3 -1.1 132.1 138.2 -17.8  MET-381   TYR-382  2.4 2.5 -24.2 19.5 85.9 80.9 21.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLU-396   PHE-397  3.7 2.6 -9.9 -8.9 72.8 68.4 18.9  PHE-397   LEU-398  5.7 4.8 -7.9 -11.9 31.4 25.1 59.1  LEU-398   GLU-399  4.9 4.5 29.1 -47.2 120.8 108.5 59.5  GLU-399   GLY-400  7.4 7.4 33.5 -17.0 113.4 128.1 -40.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees