Bifunctional P-450/nadph-P450 Reductase
(All numbering and residues are taken from first PDB file)
Bending Residue Dihedral Analysis
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
TYR-166
ARG-167
16.5
16.4
7.6
-26.5
34.2
35.4
-102.4
ARG-167
ASP-168
14.3
14.3
22.2
4.6
39.2
36.9
75.8
ASP-168
GLN-169
14.8
13.4
-39.1
22.7
113.8
89.5
-47.4
GLN-169
PRO-170
11.0
10.0
-37.6
-3.4
37.3
18.9
181.5
PRO-170
HIS-171
8.3
8.1
6.2
7.0
126.2
120.9
-35.6
HIS-171
PRO-172
7.1
7.4
0.1
6.4
65.8
70.5
-6.4
PRO-172
PHE-173
5.1
5.7
-8.8
-1.2
68.2
68.9
22.7
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
LYS-224
ALA-225
5.5
5.2
-0.6
9.6
113.2
119.7
13.6
ALA-225
SER-226
8.6
8.4
-37.4
19.4
114.0
119.9
-81.8
GLN-229
SER-230
6.8
5.2
-6.7
9.6
45.1
54.3
-36.8
SER-230
ASP-231
6.8
7.5
21.1
50.6
76.3
91.7
-54.0
ASP-231
ASP-232
7.9
9.9
-82.4
52.8
31.2
37.5
134.5
ASP-232
LEU-233
8.3
7.9
-18.4
-11.9
73.2
100.9
2.3
LEU-233
LEU-234
8.0
7.6
15.8
-0.3
79.5
72.9
21.3
LEU-234
THR-235
4.3
4.0
1.8
-11.6
18.3
4.5
-40.1
THR-235
HIS-236
4.3
4.1
-2.0
16.0
103.7
113.8
25.2
HIS-236
MET-237
7.6
7.4
-16.8
-3.9
62.1
81.1
9.6
MET-237
LEU-238
7.3
7.2
7.2
15.5
57.4
55.5
11.8
LEU-238
ASN-239
5.5
4.9
-5.9
25.8
163.0
159.7
97.3
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees