Fab Fragment, Antibody A5b7

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 VAL-104   GLU-105  3.4 3.3 -3.7 13.4 80.6 76.4 1.2
 GLU-105   VAL-106  0.5 0.9 10.9 21.5 23.8 35.9 94.8
 VAL-106   LYS-106A  3.1 3.4 -43.1 28.3 102.6 118.8 -27.8
 LYS-106A   ARG-107  5.1 4.8 6.7 -23.9 73.4 76.1 -2.5

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Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees