Pyruvate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLU-88   ILE-89  7.1 6.4 -66.1 45.7 68.7 76.4 92.3  ILE-89   ARG-90  4.6 3.9 -1.7 -13.4 119.8 117.7 -44.1  ARG-90   THR-91  7.3 7.0 -17.8 24.3 79.3 71.9 36.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-177   ASN-178  3.6 3.3 15.5 -7.7 89.2 94.6 68.5  ASN-178   LEU-179  1.4 0.8 -13.6 15.9 93.1 91.5 17.1  LEU-179   PRO-180  3.4 3.4 -13.0 -12.3 69.7 64.3 85.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  CYS-182   ASP-183  8.7 8.6 168.7 -156.7 149.7 122.8 -90.7  ASP-183   VAL-184  9.8 9.1 32.4 10.3 134.9 130.0 -91.8  VAL-184   ASP-185  6.6 6.6 -11.1 -16.9 37.2 36.2 118.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-186   PRO-187  7.4 6.3 3.8 6.9 77.8 89.4 -8.4  PRO-187   ALA-188  10.5 9.6 -13.6 -2.6 50.3 45.8 49.4  ALA-188   VAL-189  13.9 12.4 14.0 12.9 87.9 72.4 58.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees