Nad-Dependent Formate Dehydrogenase (E.C.1.2.1.2)

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ILE-122   GLY-123  11.7 11.6 3.0 -1.5 77.9 73.5 87.4  GLY-123   SER-124  14.4 14.3 -17.2 13.5 133.1 132.8 20.0  SER-124   ASP-125  17.8 17.7 22.3 -8.6 78.9 84.7 8.3  ASP-125   HIS-126  19.6 19.1 -1.8 -5.6 160.7 158.4 -55.8  HIS-126   VAL-127  19.6 19.5 -6.4 10.8 101.6 93.6 -46.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  SER-147   ILE-148  5.0 4.6 -0.5 1.3 76.5 78.4 43.1  ILE-148   SER-149  1.3 0.9 -1.7 -0.9 141.0 141.4 -27.5  SER-149   VAL-150  0.8 0.6 4.3 -1.5 90.7 90.1 50.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ILE-333   SER-334  5.4 5.4 11.3 2.3 30.7 31.7 122.4  SER-334   GLY-335  4.6 4.6 8.6 -13.3 81.1 75.0 -6.5  GLY-335   THR-336  4.3 3.8 11.1 0.6 135.2 136.9 -40.5  THR-336   THR-337  3.1 2.8 -1.0 1.2 75.2 73.7 -12.5  THR-337   LEU-338  1.1 1.5 -0.3 2.5 74.3 73.9 34.5  LEU-338   THR-339  4.7 5.0 -0.4 -1.3 143.2 141.6 -20.3  THR-339   ALA-340  5.9 6.1 -1.0 2.9 106.0 106.0 -8.1 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees