Arf Guanine-Nucleotide Exchange Factor 2

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASN-625   GLU-626  7.0 6.7 -4.6 5.7 177.2 176.9 7.2  GLU-626   TYR-627  7.0 6.7 3.0 -1.1 70.2 70.9 43.1  TYR-627   ILE-628  3.2 2.9 -3.2 -4.0 87.5 90.5 35.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ARG-643   ILE-644  13.9 14.3 3.5 1.0 132.0 135.6 -18.2  ILE-644   LEU-645  11.3 11.8 -1.5 -9.9 116.0 114.1 -55.5  LEU-645   LEU-646  8.5 8.9 -0.4 4.7 102.9 114.7 33.9  LEU-646   THR-647  10.0 10.6 -56.8 46.8 44.4 53.7 38.5  THR-647   LYS-648  10.6 12.8 -9.6 -11.5 74.3 66.0 58.1  LYS-648   PHE-649  7.2 9.6 -61.8 115.2 134.1 137.8 525.1  PHE-649   ARG-650  7.6 7.0 -32.2 5.7 139.7 140.4 -246.6  ARG-650   LEU-651  9.5 8.9 -18.0 -1.2 142.5 150.6 -175.5  LEU-651   PRO-652  8.0 7.8 -3.6 2.0 132.5 126.6 18.0  PRO-652   GLY-653  7.4 7.3 -1.5 17.8 142.8 144.1 170.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLU-654   SER-655  6.4 6.8 -7.5 -5.5 91.0 94.0 10.4  SER-655   GLN-656  4.6 5.4 15.7 -7.6 63.0 75.9 3.7  GLN-656   GLN-657  1.0 1.6 8.2 -8.8 9.0 17.3 4.2  GLN-657   ILE-658  2.2 2.2 6.6 -3.2 59.6 57.4 13.8  ILE-658   GLU-659  4.5 4.5 5.7 -3.8 89.0 93.6 -15.4  GLU-659   ARG-660  4.5 4.5 -0.6 -1.1 134.5 131.3 -13.9  ARG-660   ILE-661  3.0 3.1 5.5 -1.5 23.9 21.7 57.4  ILE-661   ILE-662  3.2 3.3 6.6 -3.2 83.5 84.3 27.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees