DNA Polymerase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  THR-473   GLU-474  8.6 6.5 -3.2 -12.2 53.5 51.3 26.7  GLU-474   ILE-475  7.8 5.5 26.8 -26.2 81.6 87.9 -5.4  ILE-475   THR-476  4.1 1.8 37.3 -39.6 34.2 37.4 -4.6  THR-476   LYS-477  4.6 2.4 19.3 -9.4 93.3 98.8 20.3  LYS-477   VAL-478  7.7 5.8 4.3 -6.4 109.9 113.4 -3.8  VAL-478   PHE-479  6.7 5.2 15.0 -15.1 51.9 52.1 -7.4  PHE-479   ASN-480  4.8 4.5 -0.7 6.0 131.6 130.4 12.8  ASN-480   GLN-481  7.6 6.8 -20.2 18.1 67.1 62.8 -5.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ILE-563   ASN-564  2.8 3.5 13.2 10.7 67.2 84.8 -1.7  ASN-564   SER-565  5.3 6.8 -20.6 26.6 158.0 150.9 11.6  SER-565   LEU-566  3.5 5.7 -23.9 5.2 86.9 87.3 29.6  LEU-566   TYR-567  2.4 4.2 35.2 -18.9 78.9 104.0 17.2  TYR-567   GLY-568  6.1 7.6 -14.6 -4.0 164.7 147.7 -42.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees