Crispr-Associated Endonuclease Cas9/csn1

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 THR-496   ASN-497  7.5 5.7 -11.9 4.7 155.4 158.1 -25.8
 ASN-497   PHE-498  7.8 6.2 -14.7 17.6 95.0 97.9 65.2
 PHE-498   ASP-499  7.8 6.5 -0.4 1.1 123.8 121.0 21.6

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 HIS-698   ASP-699  22.9 22.8 -106.5 53.5 72.2 43.2 180.5
 ASP-699   ASP-700  25.5 25.7 -165.5 -2.8 67.4 87.8 -188.8
 ASP-700   SER-701  25.6 26.7 145.8 7.4 67.8 94.9 -422.1
 SER-701   LEU-702  28.9 24.6 -3.7 38.4 145.2 143.2 178.4
 LEU-702   THR-703  27.1 25.7 162.4 -29.9 106.3 44.4 230.9
 THR-703   PHE-704  24.4 23.4 -1.3 -8.7 103.0 61.5 88.2
 PHE-704   LYS-705  21.7 21.2 22.2 -2.4 131.2 89.1 -71.6
 LYS-705   GLU-706  19.9 20.0 -0.8 7.1 71.2 124.7 -0.1
 GLU-706   ASP-707  19.4 17.6 3.7 13.2 49.8 113.6 72.5
 ASP-707   ILE-708  18.0 15.5 8.0 -27.5 76.8 136.4 59.2
 ILE-708   GLN-709  15.6 14.7 -3.4 -6.9 40.1 111.8 21.6
 GLN-709   LYS-710  14.2 15.3 -6.3 -59.1 101.9 120.3 -210.1
 LYS-710   ALA-711  13.1 12.1 36.8 -40.9 53.4 107.5 37.4
 ALA-711   GLN-712  10.4 10.2 68.9 -3.1 79.5 80.8 112.7
 ASP-718   SER-719  5.8 1.6 -122.8 -3.9 39.7 46.8 423.9
 SER-719   LEU-720  8.8 4.8 -4.6 12.4 78.9 111.1 -21.0
 LEU-720   HIS-721  9.9 7.9 -20.5 27.7 137.0 149.1 48.3
 HIS-721   GLU-722  12.0 9.6 137.5 179.6 45.8 29.1 -125.5

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees