Ribonuclease A

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  SER-21   SER-22  3.9 6.7 -16.2 56.9 55.8 13.5 -21.1  SER-22   SER-23  1.0 4.8 -158.4 -21.1 99.5 79.7 -15.2  SER-23   ASN-24  3.6 3.0 150.2 -0.4 120.4 116.0 -40.5  ASN-24   TYR-25  5.2 5.3 7.0 -1.6 67.1 62.5 3.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees