Nadph-Cytochrome P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 TYR-84   GLY-85  22.0 21.0 28.8 -31.1 74.0 74.9 -0.9
 GLY-85   SER-86  25.0 24.5 22.6 -23.5 141.4 127.2 -3.6
 SER-86   GLN-87  26.7 26.4 -28.3 29.7 79.9 73.9 0.6
 GLN-87   THR-88  30.2 30.1 10.7 -13.8 176.0 176.2 -1.5
 THR-88   GLY-89  29.8 29.6 -17.7 25.0 65.7 58.9 -2.0
 GLY-89   THR-90  26.4 26.1 -17.9 9.9 84.8 86.1 3.5
 THR-90   ALA-91  24.0 24.1 -6.1 4.2 128.8 126.1 -2.8
 ALA-91   GLU-92  22.2 22.1 -8.7 9.1 73.1 68.9 -0.6
 GLU-92   GLU-93  22.0 21.9 0.5 -2.9 134.0 134.8 0.8
 GLU-93   PHE-94  20.2 20.3 -15.3 3.9 112.0 108.5 0.5
 PHE-94   ALA-95  17.7 17.7 15.5 -8.4 61.8 56.3 1.1
 ALA-95   ASN-96  17.4 16.9 6.9 -10.1 128.4 130.8 2.4
 ASN-96   ARG-97  17.0 16.4 -3.0 12.3 67.9 64.4 -1.8
 ARG-97   LEU-98  14.0 14.1 -2.9 0.1 132.1 124.9 -0.8
 LEU-98   SER-99  12.3 11.9 -8.4 28.0 100.0 91.1 -0.1
 SER-99   LYS-100  12.9 12.0 -46.1 47.8 36.6 36.7 -1.4
 LYS-100   ASP-101  11.8 10.9 -27.8 5.6 87.4 93.9 -0.2
 ALA-102   HIS-103  7.9 8.3 -3.8 -13.6 58.5 47.8 -141.7
 HIS-103   ARG-104  9.7 5.8 0.8 29.9 115.8 68.5 15.5

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 TYR-117   ASP-118  26.8 27.0 -3.9 0.7 158.1 153.6 -5.9
 ASP-118   LEU-119  26.5 27.6 38.9 -48.1 77.9 69.8 1.9
 LEU-119   ALA-120  23.3 24.3 24.3 4.4 26.0 18.1 -187.9
 ALA-120   ASP-121  23.2 24.9 2.1 -37.7 98.7 83.0 5.3
 ASP-121   LEU-122  21.9 22.4 19.0 12.8 98.2 113.4 -5.6
 LEU-122   SER-123  18.5 19.7 -8.3 -2.3 134.3 116.3 -1.2
 SER-123   SER-124  17.7 17.3 8.6 -8.2 103.3 100.8 0.6
 SER-124   LEU-125  18.6 18.1 12.1 -15.6 140.0 160.6 0.5
 LEU-125   PRO-126  16.1 16.7 165.9 -32.7 81.3 83.9 45.7
 PRO-126   GLU-127  13.3 14.2 16.1 -47.0 90.8 134.3 3.3
 GLU-127   ILE-128  16.1 12.0 -177.5 30.4 30.4 97.3 33.8
 VAL-133   VAL-134  13.4 12.8 -15.2 36.5 75.0 72.3 -197.0
 VAL-134   PHE-135  14.2 13.2 6.4 -14.8 97.5 86.6 0.1
 PHE-135   CYS-136  17.6 16.8 1.1 1.4 126.8 120.6 -0.4
 CYS-136   MET-137  19.3 18.9 34.7 -39.8 80.0 75.6 -1.4
 MET-137   ALA-138  22.6 22.4 2.2 -0.3 109.2 101.7 -2.8
 ALA-138   THR-139  25.8 25.6 9.6 3.0 45.1 45.4 4.1

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 GLN-150   ASP-151  30.8 31.2 3.9 1.0 38.3 52.4 0.8
 ASP-151   PHE-152  30.3 29.8 -10.7 7.8 112.5 101.3 0.5
 PHE-152   TYR-153  26.5 26.1 6.6 -13.7 52.1 51.6 -3.6
 TYR-153   ASP-154  27.2 27.1 5.1 5.5 22.6 18.4 6.4
 ASP-154   TRP-155  29.5 28.7 5.2 -9.0 53.5 60.9 -2.8
 TRP-155   LEU-156  27.3 25.6 9.6 -10.6 68.6 75.2 -3.2
 LEU-156   GLN-157  24.2 23.2 4.4 -20.8 43.6 38.8 -6.5
 GLN-157   GLU-158  26.4 25.5 7.5 54.7 16.9 29.4 35.0
 GLU-158   THR-159  26.9 25.9 -50.2 41.1 101.0 109.5 -9.2
 THR-159   ASP-160  24.4 22.1 -53.8 49.5 131.0 151.2 0.8
 ASP-160   VAL-161  21.7 21.1 154.6 13.0 102.2 85.8 68.8
 VAL-161   ASP-162  20.0 17.6 11.1 153.5 97.4 98.3 69.5

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 GLY-172   LEU-173  21.5 21.1 13.2 -9.5 150.1 145.3 -2.3
 LEU-173   GLY-174  23.8 23.7 0.6 5.3 72.1 74.5 -1.2
 GLY-174   ASN-175  27.1 27.1 5.7 -7.9 129.3 129.7 2.4
 ASN-175   LYS-176  29.9 29.8 -8.1 -6.0 129.4 130.5 -4.9
 LYS-176   THR-177  31.5 31.4 12.0 -11.2 90.1 93.9 1.9
 THR-177   TYR-178  34.9 34.8 3.3 6.0 47.3 56.4 1.8
 TYR-178   GLU-179  34.8 35.0 17.1 4.0 29.5 23.5 11.0
 GLU-179   HIS-180  34.9 35.6 -5.4 -16.6 116.7 115.6 -5.4
 HIS-180   PHE-181  31.9 32.2 17.4 -2.9 129.7 127.6 -8.7
 PHE-181   ASN-182  29.4 29.6 2.4 5.4 72.5 71.4 1.2
 ASN-182   ALA-183  26.3 26.4 -0.4 2.0 162.3 156.3 1.7
 ALA-183   MET-184  27.5 27.6 1.8 3.0 81.6 81.5 -1.3
 MET-184   GLY-185  26.0 26.0 -0.2 -14.1 92.2 101.4 0.2
 GLY-185   LYS-186  22.5 22.4 18.4 -12.1 31.7 34.3 1.2
 LYS-186   TYR-187  22.4 23.2 -14.0 11.3 142.3 144.4 -1.6
 TYR-187   VAL-188  23.2 24.3 1.0 4.4 87.6 85.6 -1.1
 VAL-188   ASP-189  20.8 21.7 -1.2 -10.4 107.0 116.3 198.9
 ASP-189   GLN-190  18.0 18.8 12.7 10.9 12.8 9.1 -191.3
 GLN-190   ARG-191  18.9 20.3 -2.9 -15.7 124.7 116.4 -3.7
 ARG-191   LEU-192  19.7 20.4 18.6 -11.4 85.4 95.1 3.0
 LEU-192   GLU-193  16.8 17.2 -6.4 -6.9 129.3 131.1 -5.4
 GLU-193   GLN-194  14.3 15.0 -4.6 14.6 164.1 161.0 4.4

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 GLU-202   LEU-203  13.4 13.7 3.8 -6.7 119.0 100.7 -3.1
 LEU-203   GLY-204  16.3 16.3 5.1 -8.7 140.6 143.8 1.1
 GLY-204   LEU-205  17.5 17.7 6.4 -20.6 86.4 87.6 -2.4
 LEU-205   GLY-206  21.3 21.5 8.2 -0.9 155.7 152.6 200.0
 GLY-206   ASP-207  22.2 22.6 -8.8 11.9 87.7 95.0 -201.9
 ASP-207   ASP-208  25.7 26.2 -16.0 1.4 23.0 24.2 7.8
 ASP-208   ASP-209  27.5 27.8 -6.6 0.5 68.4 82.1 2.2
 GLY-210   ASN-211  28.1 27.8 119.9 49.3 129.7 86.9 -13.1
 ASN-211   LEU-212  24.8 24.6 -97.4 3.8 122.9 97.5 153.3
 LEU-212   GLU-213  23.0 22.7 -9.1 -7.7 66.0 67.0 -200.9
 GLU-213   GLU-214  20.3 20.0 -2.6 5.4 102.3 107.8 1.2
 GLU-214   ASP-215  19.5 19.4 -9.9 13.8 164.7 168.9 0.0
 ASP-215   PHE-216  19.6 19.6 -5.3 5.0 99.6 96.4 2.5
 PHE-216   ILE-217  17.0 16.8 2.2 0.1 113.5 107.9 -1.1
 ILE-217   THR-218  14.7 14.8 -13.7 10.2 125.3 134.4 -1.7
 ILE-217   THR-218  14.7 14.8 -13.7 10.2 125.3 134.4 -1.7

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 ILE-217   THR-218  14.7 14.8 -13.7 10.2 125.3 134.4 -1.7
 ILE-217   THR-218  14.7 14.8 -13.7 10.2 125.3 134.4 -1.7
 THR-218   TRP-219  15.1 15.3 6.4 -1.6 35.2 41.4 2.0
 TRP-219   ARG-220  14.0 14.0 1.2 -0.5 103.0 102.4 -0.5
 ARG-220   GLU-221  11.3 11.1 -23.7 21.9 76.6 89.4 -1.1
 GLU-221   GLN-222  10.1 9.9 -15.2 40.5 140.8 159.1 13.1
 GLN-222   PHE-223  9.8 10.4 -32.4 -2.8 124.8 114.7 -8.7
 PHE-223   TRP-224  8.5 8.2 27.1 -8.4 97.8 111.5 2.3
 TRP-224   PRO-225  5.3 5.3 12.0 -8.7 73.9 68.5 -1.9
 PRO-225   ALA-226  4.4 5.7 -13.2 12.8 173.2 157.0 2.5
 ALA-226   VAL-227  4.9 5.1 -0.1 -6.3 107.5 88.0 -1.8
 VAL-227   CYS-228  3.2 2.6 -6.4 25.1 80.8 76.0 1.0
 CYS-228   GLU-229  0.3 1.5 1.0 -15.1 46.8 36.3 189.9
 GLU-229   PHE-230  2.9 2.7 12.8 -6.4 30.3 56.4 -195.3
 PHE-230   PHE-231  0.7 1.0 4.4 -12.8 91.6 108.5 0.3
 PHE-230   PHE-231  0.7 1.0 4.4 -12.8 91.6 108.5 0.3

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 PHE-230   PHE-231  0.7 1.0 4.4 -12.8 91.6 108.5 0.3
 PHE-230   PHE-231  0.7 1.0 4.4 -12.8 91.6 108.5 0.3
 PHE-231   GLY-232  3.3 3.9 17.1 -19.1 112.0 105.2 187.7
 GLY-232   VAL-233  4.9 5.0 -157.5 104.6 30.3 7.0 -162.7
 VAL-233   GLU-234  6.2 5.9 -138.2 172.4 44.3 62.6 -14.1
 GLU-234   ALA-235  5.2 4.8 75.5 52.7 157.1 134.9 134.3
 ARG-243   GLN-244  14.4 21.2 -68.4 74.8 152.0 125.5 2.2
 GLN-244   TYR-245  17.2 24.4 -32.9 23.0 73.1 61.9 -0.9
 TYR-245   GLU-246  20.2 26.8 9.7 -6.9 148.2 141.3 -0.7
 GLU-246   LEU-247  21.6 27.0 2.1 11.9 127.7 142.3 -7.7
 LEU-247   VAL-248  23.2 28.0 11.1 -10.6 110.8 108.6 0.6

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees