Pyruvate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  THR-713   LYS-714  8.9 8.9 -2.3 -2.1 50.8 54.1 22.8  LYS-714   GLY-715  7.2 6.5 -23.5 0.5 68.4 66.7 33.0  GLY-715   PRO-716  3.9 3.3 11.7 -4.5 92.5 106.7 4.2  PRO-716   GLU-717  0.5 0.2 -89.0 60.8 43.2 42.4 76.3  GLU-717   ILE-718  3.5 3.4 8.6 -9.5 93.2 91.4 4.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-815   ASP-816  8.5 7.6 -39.1 30.0 22.1 21.7 44.3  ASP-816   LEU-817  9.6 8.8 69.0 -65.3 130.8 124.9 -26.3  LEU-817   PRO-818  6.2 5.4 -6.2 -13.6 58.2 63.9 37.6  PRO-818   ALA-819  5.5 4.5 -12.1 -3.2 112.9 108.6 -17.2  ALA-819   VAL-820  6.0 5.2 -1.5 -1.2 75.3 55.3 18.0  VAL-820   SER-821  3.6 4.0 -16.0 -23.3 72.0 62.0 36.0  SER-821   GLU-822  0.4 0.3 -21.4 13.1 17.2 32.4 22.5  GLU-822   LYS-823  1.9 1.4 1.0 -3.3 89.1 81.2 -3.3  LYS-823   ASP-824  5.1 4.7 -4.3 3.6 95.0 99.5 -7.8  ASP-824   ILE-825  5.0 4.8 5.4 -5.7 148.7 144.8 -5.6  ILE-825   GLN-826  4.9 5.0 -3.1 7.2 60.9 62.8 -6.2 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees