Pyruvate Kinase, M2 Isozyme

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 THR-113   LYS-114  5.5 5.0 15.6 -56.0 136.3 138.5 146.1
 LYS-114   GLY-115  6.4 5.5 122.2 -154.4 107.3 78.0 7.7
 GLY-115   PRO-116  3.4 3.6 48.1 10.9 96.7 117.9 -33.4
 PRO-116   GLU-117  5.6 6.4 -29.7 10.1 22.8 41.1 70.5

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LEU-217   PRO-218  10.3 10.6 -14.1 -3.3 49.0 64.4 41.8
 PRO-218   ALA-219  8.5 8.5 -6.9 -6.1 110.9 105.3 -3.1
 ALA-219   VAL-220  5.7 5.4 -12.9 17.4 84.7 54.5 -4.1
 VAL-220   SER-221  3.3 3.9 -42.8 -3.3 78.7 65.0 57.7
 SER-221   GLU-222  6.0 6.4 -13.2 23.6 24.3 35.9 -33.0
 GLU-222   LYS-223  7.4 7.5 -16.8 8.8 92.0 100.5 -10.7

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees