Bifunctional P-450/nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 PHE-165   TYR-166  15.4 14.7 3.8 -1.8 77.1 74.3 -6.3
 TYR-166   ARG-167  15.9 15.2 7.6 -16.8 38.5 38.8 8.8
 ARG-167   ASP-168  13.4 12.6 11.7 -4.6 37.6 36.7 -93.9
 ASP-168   GLN-169  13.6 11.3 -31.8 25.4 109.3 84.1 -7.8
 GLN-169   PRO-170  9.8 8.2 -43.2 -6.3 33.7 12.7 207.0
 PRO-170   HIS-171  7.2 6.7 10.9 13.0 128.5 119.3 -42.1
 HIS-171   PRO-172  6.4 6.4 -3.5 3.3 109.4 105.1 -16.4

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LYS-224   ALA-225  5.8 5.7 1.5 -2.9 69.2 65.6 -25.9
 ALA-225   SER-226  9.3 9.2 -17.5 1.4 109.3 109.7 -46.7
 GLN-229   SER-230  8.3 6.4 -14.2 12.0 43.4 55.6 -17.8
 SER-230   ASP-231  7.8 8.2 13.5 54.6 74.4 91.6 -54.1
 ASP-231   ASP-232  8.4 10.2 -73.3 41.1 30.7 42.0 124.1
 ASP-232   LEU-233  8.5 7.9 -18.0 -14.2 77.8 104.4 -17.4
 LEU-233   LEU-234  7.7 7.2 23.3 -12.2 76.2 70.1 14.0
 LEU-234   THR-235  4.0 3.5 2.2 -8.6 13.9 9.1 -31.7
 THR-235   HIS-236  4.0 3.8 -1.6 15.9 107.3 115.8 32.8
 HIS-236   MET-237  7.0 6.7 -12.4 -8.9 66.8 88.0 0.1
 MET-237   LEU-238  6.5 6.3 6.6 20.6 54.5 54.7 20.0
 LEU-238   ASN-239  4.4 3.7 -5.5 25.5 166.9 161.9 98.5

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees