Bifunctional P-450/nadph-P450 Reductase
(All numbering and residues are taken from first PDB file)
Bending Residue Dihedral Analysis
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
PHE-165
TYR-166
13.3
13.3
4.1
-8.5
94.5
93.5
7.6
TYR-166
ARG-167
13.7
13.4
5.7
-6.0
117.2
115.8
-38.8
ARG-167
ASP-168
10.6
10.2
-0.1
-6.8
18.0
18.3
85.5
ASP-168
GLN-169
10.1
9.1
9.3
-1.8
101.0
92.4
-15.8
GLN-169
PRO-170
6.3
5.6
-7.3
9.8
133.2
137.6
26.4
PRO-170
HIS-171
4.5
3.5
-5.3
0.7
147.8
150.7
-24.0
HIS-171
PRO-172
4.2
3.7
1.5
-2.7
83.3
79.5
11.3
PRO-172
PHE-173
1.7
2.4
1.5
-0.9
63.9
61.8
-3.2
PHE-173
ILE-174
1.6
0.8
1.1
-9.5
135.7
129.6
33.6
ILE-174
THR-175
3.8
3.0
5.8
-1.5
123.5
126.1
-3.9
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
ASP-214
LEU-215
3.4
3.4
-4.5
-2.2
92.3
92.1
29.0
LEU-215
VAL-216
0.2
0.3
-2.6
0.4
39.5
43.5
6.5
VAL-216
ASP-217
3.0
2.8
-0.8
-0.1
31.2
29.1
1.0
ASP-217
LYS-218
1.1
0.9
-4.8
-3.9
85.6
85.0
25.2
LYS-218
ILE-219
2.6
2.8
2.4
9.1
106.3
97.5
-12.2
ILE-219
ILE-220
4.5
4.8
-6.0
-0.1
9.4
14.1
20.1
ILE-220
ALA-221
3.6
4.2
4.5
-17.3
121.7
124.2
31.4
ALA-221
ASP-222
3.5
3.8
9.4
-3.0
92.1
86.0
-14.2
ASP-222
ARG-223
7.0
7.4
2.1
5.2
128.7
120.2
-22.4
ARG-223
LYS-224
8.0
8.5
-4.9
2.9
18.6
8.2
9.6
LYS-224
ALA-225
6.1
7.1
9.3
-9.1
103.3
104.7
17.0
ALA-225
SER-226
8.6
9.1
14.9
-19.9
93.2
90.5
-34.0
SER-230
ASP-231
16.4
16.7
7.6
-0.9
107.3
108.8
-3.2
ASP-231
ASP-232
17.9
18.3
-2.8
6.5
148.1
153.5
-4.3
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees