3-Deoxy-D-Manno-2-Octulosonic Acid Transferase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ILE-164   CYS-165  12.9 12.8 0.6 6.6 155.5 157.1 -208.1  CYS-165   GLN-166  13.6 13.5 -5.4 -0.9 84.9 86.5 390.5  GLN-166   LYS-167  17.1 17.0 16.3 -19.0 175.0 169.6 -172.7  LYS-167   GLY-168  16.5 16.6 -17.4 25.8 52.6 51.0 -286.8  GLY-168   LYS-169  20.0 19.9 -23.6 19.7 35.7 37.5 734.4  LYS-169   GLY-170  20.6 20.8 -13.8 5.0 50.4 54.3 480.2  GLY-170   ILE-171  17.6 18.1 27.4 -15.4 163.9 164.4 -915.9  ILE-171   LYS-172  17.6 17.8 4.3 1.5 122.7 120.4 -314.7  LYS-172   LEU-173  16.2 16.4 -1.3 -2.8 28.2 31.2 563.5  LEU-173   LYS-174  14.6 14.6 3.1 -20.6 117.4 116.6 83.2  LYS-174   GLY-175  12.7 12.5 -1.2 -3.8 84.5 71.9 -400.6  GLY-175   GLU-176  9.2 9.0 -9.9 15.8 133.9 144.4 -97.3  GLU-176   PHE-177  6.2 6.2 12.2 1.8 67.8 74.1 9.4  PHE-177   ILE-178  7.6 7.7 10.5 -11.3 35.8 35.8 -34.6  ILE-178   VAL-179  7.8 7.9 -1.7 -2.8 104.9 106.1 616.3  VAL-179   ALA-180  10.8 10.9 -2.9 -3.1 129.8 130.5 -379.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-253   TYR-254  15.4 15.3 -2.2 2.5 83.2 79.7 -173.1  TYR-254   PRO-255  12.0 12.0 1.6 -2.4 70.4 70.6 -171.9  PRO-255   VAL-256  11.4 11.6 -5.4 9.5 162.9 162.6 325.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LYS-258   ILE-259  4.1 4.2 1.6 -4.7 40.4 40.1 -14.7  ILE-259   ALA-260  4.7 4.7 -5.1 0.7 97.7 98.9 237.8  ALA-260   ILE-261  8.0 8.0 4.1 -5.9 71.1 70.0 -79.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-275   GLU-276  10.0 9.8 0.9 -1.9 39.3 40.9 98.6  GLU-276   PRO-277  12.0 12.0 -2.9 1.2 134.5 134.3 -286.3  PRO-277   THR-278  9.4 9.5 2.2 -4.3 114.5 110.4 -1.6  THR-278   CYS-279  6.8 6.9 0.9 -3.5 105.7 107.0 -96.6  CYS-279   TRP-280  9.9 9.9 14.9 -11.4 41.6 39.0 325.4  TRP-280   GLY-281  10.1 10.1 -2.5 -1.3 104.0 106.3 -198.7  GLY-281   ILE-282  7.1 7.1 -1.3 5.2 125.8 127.2 133.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  THR-313   GLU-314  17.3 17.2 2.4 4.8 123.5 123.9 -9.9  GLU-314   LEU-315  15.1 15.1 2.5 -0.3 76.8 73.6 282.2  LEU-315   VAL-316  12.8 12.8 -2.3 0.6 143.4 142.5 -14.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LYS-326   LYS-327  5.0 4.9 4.5 -7.4 7.3 7.6 -301.6  LYS-327   GLU-328  4.1 4.1 -9.6 14.5 114.8 117.2 332.2  GLU-328   ILE-329  5.8 5.9 -2.2 6.5 140.7 138.8 -106.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLU-332   GLU-333  10.3 10.3 4.3 3.6 133.3 131.3 -324.1  GLU-333   LYS-334  11.2 11.1 -4.6 2.2 21.0 24.1 157.9  LYS-334   SER-335  8.8 8.8 7.4 -13.4 108.7 106.3 369.3  SER-335   ARG-336  11.0 11.1 7.6 -3.3 110.6 112.5 -366.1  ARG-336   GLU-337  14.2 14.2 -0.3 1.3 24.7 21.8 -180.4  GLU-337   ILE-338  12.7 12.6 -6.9 1.5 43.1 42.6 339.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees