Bifunctional P-450/nadph-P450 Reductase
(All numbering and residues are taken from first PDB file)
Bending Residue Dihedral Analysis
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
PHE-165
TYR-166
17.0
16.7
8.4
-4.4
70.6
75.7
20.4
TYR-166
ARG-167
17.1
17.0
8.5
-14.7
28.7
35.6
-18.7
ARG-167
ASP-168
14.8
14.7
15.6
4.9
48.2
38.0
-11.8
ASP-168
GLN-169
15.6
13.9
-46.4
26.3
112.2
90.1
-49.4
GLN-169
PRO-170
11.9
10.4
-36.5
-5.4
34.6
14.6
176.4
PRO-170
HIS-171
9.5
8.8
11.0
6.6
117.4
113.2
-28.3
HIS-171
PRO-172
7.8
7.6
-7.0
15.4
117.4
109.9
-16.3
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
SER-230
ASP-231
6.5
6.7
45.8
1.7
69.3
70.5
8.7
ASP-231
ASP-232
6.5
7.1
-43.9
37.6
40.3
30.8
50.3
ASP-232
LEU-233
7.0
7.1
4.2
-15.5
106.7
98.2
-14.7
LEU-233
LEU-234
6.7
6.6
15.3
-5.8
92.4
96.3
-32.3
LEU-233
LEU-234
6.7
6.6
15.3
-5.8
92.4
96.3
-32.3
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
LEU-233
LEU-234
6.7
6.6
15.3
-5.8
92.4
96.3
-32.3
LEU-233
LEU-234
6.7
6.6
15.3
-5.8
92.4
96.3
-32.3
LEU-234
THR-235
2.9
2.8
0.8
-22.6
155.2
162.3
113.5
THR-235
HIS-236
2.3
2.3
6.1
14.8
107.7
114.0
-53.7
HIS-236
MET-237
5.6
5.7
-8.7
-7.4
121.9
105.5
-44.7
MET-237
LEU-238
5.9
6.0
-0.2
21.9
65.9
64.1
10.5
LEU-238
ASN-239
4.7
4.2
1.7
13.1
163.2
162.0
-65.2
ASN-239
GLY-240
4.9
4.9
7.4
6.6
113.8
98.7
-40.6
GLY-240
LYS-241
7.6
8.5
-1.3
-5.0
86.6
81.0
-24.6
LYS-241
ASP-242
11.0
11.4
4.5
-7.8
89.8
80.0
-37.3
ASP-242
PRO-243
14.3
15.1
-7.3
20.9
21.8
25.8
-73.7
PRO-243
GLU-244
15.7
16.9
-34.9
11.8
89.1
74.6
31.5
GLU-244
THR-245
19.1
19.7
-32.4
54.8
85.3
88.6
23.9
THR-245
GLY-246
18.8
18.4
-12.2
1.3
66.6
56.7
36.3
GLY-246
GLU-247
15.4
15.1
25.3
-48.3
42.1
56.9
-84.4
GLU-247
PRO-248
15.1
13.5
15.2
-4.8
63.6
46.9
42.3
PRO-248
LEU-249
11.9
10.9
11.2
-3.2
16.9
21.9
37.2
LEU-249
ASP-250
11.7
11.2
3.2
1.5
75.3
78.3
33.1
ASP-250
ASP-251
13.1
11.5
-2.6
6.1
132.7
133.6
20.3
ASP-251
GLU-252
11.2
9.0
14.9
-15.8
22.8
17.1
1.4
GLU-252
ASN-253
12.6
10.4
7.4
-0.7
90.2
83.4
6.6
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees