Pyruvate Kinase Isozymes M1/m2

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-116   PRO-117  9.4 9.2 13.7 -2.2 109.7 102.4 -18.9  PRO-117   GLU-118  6.2 5.6 -10.0 35.9 131.5 130.0 88.3  GLU-118   ILE-119  4.3 4.5 -18.0 0.5 63.4 64.2 30.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-209   ASN-210  3.8 3.8 18.1 -8.7 90.5 90.5 25.0  ASN-210   LEU-211  1.9 2.3 9.1 3.2 84.6 80.7 12.6  LEU-211   PRO-212  5.1 5.6 -9.4 4.7 76.0 68.7 15.1  PRO-212   GLY-213  6.9 7.3 8.4 -7.7 68.3 68.4 20.9  GLY-213   ALA-214  10.3 10.8 13.0 -19.3 12.0 10.2 -32.7  ALA-214   ALA-215  9.1 9.8 24.9 -18.6 55.8 66.2 -18.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-216   ASP-217  6.0 6.2 -13.8 17.1 133.4 131.1 42.3  ASP-217   LEU-218  6.3 6.6 -25.0 22.3 104.2 109.5 19.9  LEU-218   PRO-219  5.1 4.6 4.1 3.4 83.6 88.9 15.3  PRO-219   ALA-220  8.3 7.9 -10.8 -1.5 40.6 36.3 22.8  ALA-220   VAL-221  11.1 10.2 14.1 -1.3 85.4 76.1 27.5  VAL-221   SER-222  12.0 11.5 -5.3 -5.2 134.3 140.5 -31.8  SER-222   GLU-223  9.6 9.4 -1.0 -2.7 125.3 122.5 11.7  GLU-223   LYS-224  10.8 11.1 5.9 2.8 122.9 122.4 -3.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees