Pyruvate Kinase, M2 Isozyme

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 THR-113   LYS-114  9.4 9.1 14.5 -19.5 124.2 117.5 3.0
 LYS-114   GLY-115  7.6 6.6 -11.2 -13.7 78.9 77.7 21.0
 GLY-115   PRO-116  4.3 3.5 24.0 -0.9 83.7 98.8 4.2
 PRO-116   GLU-117  0.6 0.1 -69.5 29.1 33.1 36.5 80.1
 GLU-117   ILE-118  3.1 3.0 -8.9 11.7 102.0 99.6 5.5

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 VAL-215   ASP-216  8.9 8.3 -25.3 2.2 30.8 45.4 42.6
 ASP-216   LEU-217  9.4 8.2 69.8 -55.0 130.0 129.1 -42.1
 LEU-217   PRO-218  6.0 4.8 9.2 -28.9 118.7 111.8 7.7
 PRO-218   ALA-219  5.0 4.0 -3.3 -2.2 123.6 114.7 10.4
 ALA-219   VAL-220  5.9 4.9 3.2 -2.4 90.9 119.3 4.7
 VAL-220   SER-221  3.9 3.7 -32.1 -6.5 68.0 58.2 29.9
 SER-221   GLU-222  1.8 1.2 -9.1 12.6 31.3 40.8 2.3

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees