Mitogen-Activated Protein Kinase 14

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-73   LEU-74  4.2 3.7 -5.6 6.3 26.1 26.5 -8.1  LEU-74   LYS-75  3.4 3.1 -3.7 5.7 85.9 85.5 -35.5  LYS-75   HIS-76  1.4 1.7 -3.6 6.2 113.4 115.2 81.1 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-84   LEU-85  5.2 5.1 15.7 -3.3 58.7 59.2 68.2  LEU-85   LEU-86  1.6 1.4 7.2 -4.8 48.6 43.9 69.7  LEU-86   ASP-87  1.7 1.6 3.9 -4.0 94.0 89.6 12.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  THR-105   HIS-106  3.9 4.0 -12.4 11.4 24.5 29.1 21.0  HIS-106   LEU-107  5.7 5.8 -1.5 -15.7 75.6 73.9 130.5  LEU-107   MET-108  7.9 8.0 -9.0 5.3 91.4 82.3 98.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASP-320   PRO-321  11.1 11.0 1.0 -7.5 133.8 128.7 -55.0  PRO-321   TYR-322  9.2 9.3 -3.5 13.4 107.0 103.2 116.6  TYR-322   ASP-323  5.7 5.9 -2.6 -3.3 36.2 40.3 59.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees