Pyruvate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LEU-82   ASP-83  16.7 16.7 1.3 -4.9 53.4 56.7 -29.9
 ASP-83   THR-84  13.9 14.1 -16.4 12.5 63.9 67.0 13.2
 THR-84   LYS-85  10.6 10.8 -4.6 -8.8 120.1 122.8 -21.6
 LYS-85   GLY-86  7.7 8.6 4.4 9.7 67.5 74.1 25.0
 GLY-86   PRO-87  5.4 5.5 -15.3 11.1 94.6 88.3 -0.9
 PRO-87   GLU-88  1.8 1.9 61.8 -5.1 36.6 35.9 135.6
 GLU-88   ILE-89  2.8 3.5 -15.2 12.4 82.3 89.1 -16.5

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 VAL-184   ASP-185  6.7 6.9 6.6 -26.2 34.7 33.2 -41.9
 ASP-185   LEU-186  7.3 7.9 -60.0 73.7 125.0 143.2 39.3
 LEU-186   PRO-187  3.9 4.8 8.3 7.0 63.7 63.4 -19.2
 PRO-187   ALA-188  4.3 5.4 15.4 -13.1 110.6 114.3 -11.6
 ALA-188   VAL-189  6.5 6.8 7.2 8.9 65.8 71.9 60.2
 VAL-189   SER-190  5.7 5.5 12.1 4.8 53.4 65.7 22.4
 SER-190   ALA-191  2.4 2.6 -8.0 2.1 148.3 157.3 -15.7
 ALA-191   LYS-192  2.9 3.1 -0.4 2.8 80.6 87.5 6.4
 LYS-192   ASP-193  6.1 6.6 -0.3 -2.6 76.8 77.5 2.3
 ASP-193   ARG-194  7.0 7.4 2.3 4.9 37.6 33.6 13.7
 ARG-194   VAL-195  7.7 7.3 2.2 -12.4 64.7 58.2 -0.4
 VAL-195   ASP-196  9.2 9.2 24.0 -11.3 121.0 129.2 -23.8
 ASP-196   LEU-197  11.6 11.1 -1.0 -0.1 91.8 93.1 0.8

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees