Pyruvate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LEU-82   ASP-83  17.0 17.0 -2.9 0.8 129.4 122.4 -29.0
 ASP-83   THR-84  14.3 14.3 -14.7 12.7 66.2 67.3 15.7
 THR-84   LYS-85  11.0 11.1 -11.3 2.6 121.0 123.5 -16.8
 LYS-85   GLY-86  8.1 9.0 7.1 19.7 73.2 75.5 31.2
 GLY-86   PRO-87  5.6 5.8 -18.5 2.7 105.8 88.5 -24.2
 PRO-87   GLU-88  2.1 2.3 71.5 -10.9 38.0 34.6 147.5
 GLU-88   ILE-89  3.1 3.8 -18.4 18.3 84.6 88.9 -16.8

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 VAL-177   ASN-178  6.8 6.6 7.9 8.3 95.2 97.7 28.5
 ASN-178   LEU-179  3.7 3.8 34.9 -17.6 110.3 105.4 -4.7
 LEU-179   PRO-180  5.0 5.5 -4.9 -2.6 74.1 56.8 -43.4
 PRO-180   GLY-181  4.4 5.1 -157.7 164.7 86.7 87.0 9.0
 GLY-181   CYS-182  8.0 8.8 50.6 -51.2 36.2 22.9 26.1
 CYS-182   ASP-183  8.6 9.0 -3.5 0.0 138.5 128.2 6.0
 ASP-183   VAL-184  8.9 9.3 -1.5 27.4 135.2 142.7 56.6
 VAL-184   ASP-185  6.2 6.6 60.4 -54.4 42.4 32.1 20.0
 ASP-185   LEU-186  6.7 7.5 -54.5 43.5 132.0 141.6 -22.8
 LEU-186   PRO-187  3.4 4.3 3.7 -2.9 61.0 62.6 -42.0
 PRO-187   ALA-188  3.8 4.9 16.7 -9.2 111.8 114.9 -15.2
 ALA-188   VAL-189  6.1 6.5 0.9 11.0 63.7 73.4 64.3
 VAL-189   SER-190  5.5 5.4 9.3 11.6 53.9 66.6 22.8
 SER-190   ALA-191  2.5 2.9 2.3 -3.5 35.7 24.0 -0.5
 ALA-191   LYS-192  3.3 3.7 0.1 -5.8 103.2 93.6 -3.5
 LYS-192   ASP-193  6.4 7.2 5.2 0.9 102.9 101.7 2.1
 ASP-193   ARG-194  7.1 7.8 4.5 0.5 37.2 32.5 18.3
 ARG-194   VAL-195  7.8 7.6 -4.8 -7.4 113.2 120.5 -5.3
 VAL-195   ASP-196  9.4 9.6 23.9 -14.3 122.0 129.6 -20.6
 ASP-196   LEU-197  11.7 11.5 1.9 -0.4 85.2 85.9 2.4
 LEU-197   GLN-198  12.8 12.5 0.5 2.0 31.7 33.5 4.3

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees