RNA Polymerase Sigma-70 Subunit

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 GLY-180   GLU-181  8.4 8.4 0.6 0.0 148.4 147.8 0.9
 GLU-181   ALA-182  11.1 11.1 -1.0 0.4 79.3 78.6 -1.6
 ALA-182   ALA-183  13.1 13.1 -0.5 -0.9 71.8 70.9 12.8
 ALA-183   ARG-184  10.5 10.5 1.7 -7.0 170.3 169.2 88.3
 ARG-184   GLN-185  9.5 9.5 -27.4 27.8 55.8 54.5 25.8
 GLN-185   HIS-186  13.1 13.0 -2.9 5.2 89.2 88.4 -90.5
 HIS-186   LEU-187  14.0 13.9 -6.5 4.4 53.7 51.3 -12.0
 LEU-187   ILE-188  11.3 11.1 -3.6 7.2 17.6 18.3 -69.3

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Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees