Bifunctional P-450/nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 GLU-4   MET-5  11.2 10.6 -30.2 31.1 70.2 73.9 75.4
 MET-5   PRO-6  7.4 6.9 -0.2 -0.4 101.9 109.7 32.6
 PRO-6   GLN-7  6.3 6.3 2.4 10.5 46.0 49.8 39.5
 GLN-7   PRO-8  5.1 5.0 0.1 -8.1 79.9 84.6 -0.9
 PRO-8   LYS-9  6.8 7.0 13.8 -5.0 27.8 23.7 61.0

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LEU-17   PRO-18  8.0 7.6 -3.6 -7.3 97.4 99.3 -6.2
 PRO-18   LEU-19  11.2 10.9 5.7 23.1 17.5 18.6 142.3
 LEU-19   LEU-20  10.9 10.8 -24.4 43.2 111.4 106.2 26.3
 LEU-20   ASN-21  11.4 11.9 -41.2 2.7 54.5 63.0 92.2
 ASN-21   THR-22  14.6 14.9 -3.2 0.1 129.3 127.1 -50.2
 THR-22   ASP-23  16.7 17.1 -6.5 29.0 94.8 112.2 94.2
 ASP-23   LYS-24  18.6 19.8 -28.9 -8.3 164.3 149.1 -136.1
 LYS-24   PRO-25  17.1 17.7 -0.4 6.4 91.1 87.0 -5.7
 PRO-25   VAL-26  13.4 14.1 -6.9 -8.0 147.3 147.8 -18.7

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 GLU-38   ILE-39  1.1 1.2 -2.7 2.2 146.8 146.5 -13.8
 ILE-39   PHE-40  2.0 1.8 -3.0 7.6 139.4 142.9 33.6
 PHE-40   LYS-41  1.5 1.5 2.7 0.4 37.9 38.6 12.5

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 GLY-46   ARG-47  5.6 5.9 41.9 -17.7 28.3 26.0 161.8
 ARG-47   VAL-48  5.7 6.4 -9.6 -2.1 137.6 133.4 -111.3
 VAL-48   THR-49  4.6 5.0 10.3 10.7 35.0 34.3 66.8
 THR-49   ARG-50  5.5 5.6 1.5 -6.0 42.7 47.1 -40.4

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees