Interstitial Collagenase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  THR-255   PRO-256  1.2 0.5 9.5 -9.1 36.8 43.8 3.6  PRO-256   LYS-257  2.6 2.9 -3.5 1.0 135.7 142.6 -8.3  LYS-257   ALA-258  2.4 2.9 -3.5 -23.2 112.3 112.9 -76.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PHE-282   TYR-283  5.4 6.4 -21.9 35.3 77.6 77.8 -18.5  TYR-283   MET-284  8.9 9.9 -1.6 0.2 27.5 20.4 41.4  MET-284   ARG-285  10.3 10.8 25.6 -47.2 96.6 102.2 87.2  ARG-285   THR-286  14.0 14.2 20.6 57.7 160.2 146.1 -519.5  THR-286   ASN-287  15.6 15.6 29.7 -84.1 94.2 72.1 -56.5  ASN-287   PRO-288  19.0 17.2 -7.5 0.1 29.9 33.0 48.8  PRO-288   PHE-289  20.9 18.9 -11.4 3.4 47.3 87.0 28.3  PHE-289   TYR-290  22.0 18.1 86.2 -19.2 119.4 93.4 -161.3  TYR-290   PRO-291  19.6 14.3 33.6 -2.2 73.9 145.8 -98.9  PRO-291   GLU-292  15.9 12.7 -173.2 29.7 97.7 93.4 -367.6  GLU-292   VAL-293  13.8 10.6 -7.6 126.8 119.4 152.4 797.5  VAL-293   GLU-294  11.7 11.1 68.2 -7.5 40.0 47.9 273.8  GLU-294   LEU-295  11.8 12.1 -16.9 7.2 119.5 125.6 -56.4  LEU-295   ASN-296  8.6 8.9 -2.5 -4.0 142.0 138.2 -31.8  ASN-296   PHE-297  8.2 9.0 -9.0 12.9 135.8 142.0 53.9  PHE-297   ILE-298  6.5 7.8 -7.8 9.1 120.2 114.2 6.8  ILE-298   SER-299  8.2 9.4 -2.4 2.5 142.0 139.6 18.2  SER-299   VAL-300  8.0 9.4 17.9 9.3 101.3 99.4 -3.5  VAL-300   PHE-301  10.5 12.3 -11.3 13.9 85.0 110.6 42.0  PHE-301   TRP-302  12.9 14.5 -28.3 4.0 138.0 158.8 -126.1  TRP-302   PRO-303  12.5 12.9 12.4 -30.8 43.6 67.6 -136.6  PRO-303   GLN-304  11.0 10.1 23.4 8.3 46.1 44.3 180.2  GLN-304   LEU-305  10.8 10.1 -14.8 21.7 72.4 67.8 -19.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees