Pyruvate Kinase, M2 Isozyme

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 THR-113   LYS-114  9.5 9.5 9.8 -11.3 123.4 113.5 9.6
 LYS-114   GLY-115  7.8 7.1 -28.2 11.7 79.5 72.0 15.5
 GLY-115   PRO-116  4.4 3.7 27.0 -16.3 82.8 90.2 8.7
 PRO-116   GLU-117  0.9 0.3 -68.3 28.5 33.9 35.5 89.8
 GLU-117   ILE-118  3.4 3.3 -8.7 9.2 102.9 101.1 -3.1

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LEU-217   PRO-218  5.7 4.7 -2.5 -24.1 62.1 63.0 28.1
 PRO-218   ALA-219  4.7 3.4 -11.8 -5.2 124.5 112.0 -23.8
 ALA-219   VAL-220  5.6 4.3 -2.0 -1.8 89.6 64.9 -5.9
 VAL-220   SER-221  3.7 3.7 -62.0 39.4 67.3 56.3 40.1
 SER-221   GLU-222  2.1 2.0 -12.2 -1.2 32.1 31.2 32.6

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees