Bifunctional P-450/nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 PRO-18   LEU-19  11.1 10.8 5.6 23.5 22.6 23.2 162.4
 LEU-19   LEU-20  11.2 10.9 -20.7 33.5 117.0 113.0 34.3
 LEU-20   ASN-21  11.6 11.9 -35.6 6.0 55.6 59.9 118.5
 ASN-21   THR-22  14.7 14.8 -1.2 -1.0 128.9 123.2 -69.9
 THR-22   ASP-23  16.9 17.2 -5.6 17.9 101.8 115.1 40.1
 ASP-23   LYS-24  18.7 19.6 -18.0 -4.1 169.0 158.3 -97.4
 LYS-24   PRO-25  17.2 17.6 -2.1 9.3 90.7 89.0 12.6
 PRO-25   VAL-26  13.5 14.0 -9.1 -5.1 150.1 148.1 -41.7
 VAL-26   GLN-27  12.6 12.9 7.7 -4.4 63.2 62.6 33.1

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LEU-36   GLY-37  8.1 8.2 0.9 4.2 137.7 136.1 -24.4
 GLY-37   GLU-38  6.2 6.3 -2.8 2.6 93.0 90.2 18.9
 GLU-38   ILE-39  2.7 3.0 -1.6 2.1 139.5 139.5 6.7

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ARG-50   TYR-51  3.2 3.1 4.4 -2.4 35.7 39.7 2.1
 TYR-51   LEU-52  2.9 2.9 -3.8 7.2 149.5 148.7 15.4
 LEU-52   SER-53  2.5 2.4 -3.2 5.6 131.1 132.5 -20.6

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees